Development of Gateway vectors for plant research
We are developing a Gateway cloning technology-compatible vector construction system and continue to provide various destination vectors, which are useful in plant research, as one of joint research project. Please read the references as follows.
[For BiFC experiments]
Kamigaki, A., Nito, K., Hikino, K., Goto-Yamada, S., Nishimura, M., Nakagawa, T., and Mano, S. (2016). Gateway vectors for simultaneous detection of multiple protein−protein interactions in plant cells using bimolecular fluorescence complementation. PLOS ONE 11, e0160717. [open access]
Tanaka, Y., Kimura, T., Hikino, K., Goto, S., Nishimura, M., Mano, S., and Nakagawa, T. (2012). Gateway vectors for plant genetic engineering: overview of plant vectors, application for bimolecular fluorescence complementation (BiFC) and multigene construction. In Genetic Engineering – Basics, New Applications and Responsibilities – Edited by Hugo A. Barrera-Saldaña, InTech, pp.35-58. [open access]
[For generation of transgenic Marchantia polymorpha]
Mano, S., Nishihama, R., Ishida, S., Hikino, K., Konodo, M., Nishimura, M., Yamato, T.K., Kohchi, K., and Nakagawa, T. (2018) Novel gateway binary vectors for rapid tripartite DNA assembly and promoter analysis with various reporters and tags in the liverwort Marchantia polymorpha. PLOS ONE, 13(10), e0204964. [open access]
Almost all the vectors are available for non-commercial research purposes, although the permission of original developers is required for some tags/reporters.
The information on the vectors for BiFC experiments and generation of transgenic M. polymorpha are shown in the following pages.
• For BiFC experiments
• For generation of transgenic M. polymorpha
The information of another types of vector sets, such as pGWB, R4pGWB, R4L1pGWB series, is shown in the website of Prof. Nakagawa in Shimane University.